At resting membrane potential, rhodopsin absorbance is low and therefore fluorophore emission is high. We were not able to quantitatively measure these spectra in cell membranes at different voltages. Spectra 14 in the lower panels have been scaled to illustrate the direction of absorbance and fluorescence change with membrane voltage change. b Schematic representing the proposed mechanism of negative-going rhodopsin eFRET GEVIs. Photocurrent measurements (bottom) for the Ace2 rhodopsin proton pump. The proton acceptor (PA), proton donor (PD), and proton release (PR) positions are represented as yellow spheres and labeled, retinal is shown as blue sticks. 1a, b).Ī Schematic (top) showing the hypothetical path of proton transport (arrows) through the Ace2 rhodopsin proton pump. 1c) and other rhodopsin-based GEVIs 21, combined with previous mutagenesis and biochemical data 17, 20, suggest that voltage sensitivity in Ace2 D81N and other eFRET GEVIs results from membrane potential changes altering the equilibrium of protonation between the retinal Schiff base, the proton donor (PD) residue 20, and the cell cytoplasm (Fig. Electrophysiology measurements showing transient inward photocurrents with Ace2 D81N (Fig. This mutation blocks the primary pathway for exchange of protons from the retinal Schiff base, which links retinal to the rhodopsin protein, to outside the cell 20. 1a, b), analogous to the Arch D95N mutation described above. 1a) is blocked by mutating the residue that normally functions as the proton acceptor (PA) 20 (D81N) (Fig. In both of these GEVIs, photocurrent of Ace2 rhodopsin (Fig. We recently used the same Ace2 rhodopsin to engineer a negative-going chemigenetic eFRET GEVI called Voltron, which uses a HaloTag protein domain to covalently bind bright and photostable small-molecule flurophores 18, 19, extending the duration and number of neurons imaged simultaneously in vivo 14. Previous work fused the Ace2 rhodopsin from Acetabularia acetabulum 17 to the FP mNeonGreen to produce a negative-going eFRET GEVI that allowed in vivo imaging of voltage signals in several model organisms 12. Here we present a general approach to engineer eFRET GEVIs with fast, bright, and positive-going fluorescence signals in response to neuronal action potentials by modification of the natural proton transport pathway within microbial rhodopsins. Although two VSD GEVIs, FlicR 4 and Marina 16, exhibit positive-going signals in neurons, they have significantly slower response kinetics than eFRET GEVIs, making detection of action potentials difficult. All reported eFRET GEVIs therefore have negatively sloped fluorescence-voltage relationships they are brighter at resting membrane potential and become dimmer during an action potential (negative going). Although the absorbance of the retinal cofactor increases with increasing membrane potential, the emission of the eFRET-coupled fluorophore consequently decreases. These fusions enable voltage-sensitive electrochromic fluorescence resonance energy transfer (eFRET) from a bright fluorophore to the retinal cofactor within the rhodopsin, which acts as a dark quencher. Fusions of fluorescent protein (FP) domains or other bright fluorophores to rhodopsin GEVIs were therefore made to facilitate imaging 11, 13, 14. However, rhodopsin fluorescence is very dim, requiring intense illumination for imaging 8. The rhodopsin GEVI optical signal is fast and linear 8, two desirable features for a voltage indicator. The equivalent mutation in Ace1 12, Ace2 12, 14, and Mac 13 rhodopsins was used to generate later GEVIs. A single amino acid substitution (D95N) in Arch abolished light-driven currents and retained Arch voltage-dependent fluorescence change 8. However, Arch also generated a hyperpolarizing light-driven current upon exposure to imaging light 8, 15 by functioning as an outward proton pump 15. Archaerhodopsin 3 (Arch) from Halorubrum sodomense was the first rhodopsin GEVI that accurately tracked changes in neuronal membrane potential 8. Existing GEVIs use voltage-sensitive protein domains from voltage-sensitive ion channels or phosphatases 3, 4, 5, 6, 7 (VSD GEVIs), or microbial rhodopsin domains 8, 9, 10, 11, 12, 13, 14 (rhodopsin GEVIs). Knowledge of the mechanistic function of GEVIs will lead to improved designs. Despite recent advances, no current GEVI has ideal properties for routine, robust in vivo imaging. Monitoring voltage signals in vivo in large populations of neurons will enable dissection of detailed mechanistic links between brain activity and animal behavior. Genetically encoded voltage indicators (GEVIs) allow visualization of fast action potentials and subthreshold dynamics in groups of genetically defined neurons with high spatiotemporal resolution 1, 2.
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Holm, Invisible Emmie is a humorous and surprising debut graphic novel by Terri Libenson, creator of the internationally syndicated, Reuben Award-winning comic strip The Pajama Diaries. Can Emmie find the courage to speak up and find her inner Katie? Or will she choose to remain invisible? Told in text interspersed with graphic-style illustrations, “Invisible Emmie” is a smart, funny story that will appeal to younger fans of Raina Telgemeier and “Roller Girl. Invisible Emmie Libenson, Terri Lexile & Reading Level: Synopsis: Perfect for fans of Raina Telgemeier and Jennifer L. As their lives intersect, they might just find they have more in common then they thought. Un roman illustré plein dhumour Cest lhistoire de deux filles que tout sépare : Emmie, timide, solitaire et passionnée de dessin Katie, extravertie, populaire et sportive. When an inside joke between Emmie and her best friend Brianna goes horribly, humiliatingly wrong, Katie steps in to help. Perfect for fans of Raina Telgemeier and Jennifer L. Questions focus on:Comprehension StrategiesDemonstrating Unders. Then there’s Katie, who’s everything Emmie is not: popular, outgoing, athletic. Browse invisible emmie resources on Teachers Pay Teachers, a marketplace trusted by. First there’s our narrator, Emmie, who’s shy, artistic, and so quiet at school she seems to disappear, often wishing she had a pile of books to bury her face in. In Terri Libenson’s “ Invisible Emmie,” we get a glimpse into the lives of two very different middle school girls. I’m hoping to return to human form soon.” I never thought I was much to look at to begin with, but a puddle of slime really isn’t very attractive. It involved a really embarrassing incident. Okay, five hours, if you want to get technical. A highly relatable middle grade drama." - School Library Journal "Libenson's clever tale will entertain readers in the throes of middle school as well as younger students both wary of and intrigued by their near future.“You’re probably wondering how I became a puddle of slime. A well-executed twist will have readers flipping back to see what they missed while cheering the strides made by Libenson's no-longer-invisible heroine." - Publishers Weekly "With all-too-familiar middle-school drama and an empowering lesson about speaking up and bravely facing down embarrassment, this should find an easy audience among fans of Wimpy Kid or Dork Diaries books." - Booklist "Many readers will recognize themselves in Emmie and her friends, who are at once self-conscious and eager to be seen for who they are. Reading Invisible Emmie sums up middle school: You laugh, you cry, you get beaned in the head with a volleyball." - Stephan Pastis, author of Timmy Failure "In her first children's book, cartoonist Libenson offers strikingly different visions of seventh grade through two very dissimilar narrators. Invisible Emmie is unforgettable!" - Lincoln Peirce, author of Big Nate "This funny and heartfelt tale will ring true for anyone who's ever felt invisible." - Victoria Jamieson, Newbery Honor author-illustrator of Roller Girl "Clever, funny work by a great cartoonist. A fantastic debut novel with plenty of laughs and tons of heart. "This is middle grade fiction at its best. 1.4 “You” or “Your” means you or you and your employer and its affiliates, whether or not capitalized. 1.3 “Materials” means software or other collateral Intel delivers to You under this Agreement. 1.2 “Intel Components” means a hardware component or product designed, developed, sold, or distributed by Intel or its affiliates. 1.1 “Including” means including but not limited to, whether or not capitalized. If You do not agree, do not use the Materials and destroy all copies. By downloading, installing, or using the Materials, You agree to these terms. If You are accepting this Agreement on behalf of or in conjunction with Your work for Your employer, You represent and warrant that You have the authority to bind your employer to this Agreement. It is available for free and provides access to unlimited driver updates and software installations.Deprecated: Intel OBL Distribution (Commercial Use) License This LIMITED DISTRIBUTION LICENSE AGREEMENT (“Agreement”) is a contract between You and Intel Corporation and its affiliates (“Intel”). It is suitable for both beginners and advanced users due to its versatility in features and modes. However, the size of drivers it downloads can vary. It is a fast working computer application that does not need much space on hard disk. Here the user can choose general and advanced tool settings like application language, temporary file removal, activating desired modes, restore point or backup creation, driver selection and can customize software layout. It is often preferred by system administrators as it automates the process of setting up the systems. Personalization:ĭriverPack Solution allows you to customize the working of the software through Settings option. It contains useful information such as system hardware summary, temperature of certain components, motherboard specifications, display devices, multimedia, storage, partitions, input terminals, network adapters, peripheral devices, battery and power management properties, operating system properties and security status. Diagnostics:ĭiagnostics section shows all the details of your system. It lets you know of the software on your PC that are recommended for removal, can be deleted or can be kept and thus assists users in removing unwanted programs. It can be considered as an advanced form of anti-malware tool because it is capable of detecting those virus, adware and unwanted software programs that can harm PCs. It displays all the applications that are either useless or contain malware. System Security:ĭriverPack Solution helps you improve the security of your system with the help of DriverPack Protect functionality. It is capable of detecting all types of drivers for Bluetooth, Video Card, Graphic Card, Sound Card, Network Interface, Wi-Fi, Processor and Input or Output devices. Installing the updates can improve the performance however, it can slow down your PC if enough space is not available in system memory. Either you can install all the recommendations by clicking Install Everything button or choose programs individually. The Drivers tab in DriverPack Solution Online displays the list of all types of drivers and utilities that are installed on your system and need to be updated. This feature is very useful as it helps users save a lot of time and efforts. It can automatically scan your system, find the outdated drivers and can update them to improve system performance. Online edition cannot work without internet connection, whereas Offline edition does not require internet to operate. It is available in two different versions. It is a platform through which you can find out which of the existing drivers need to be updated while which applications should be installed. DriverPack Solution is a smart computer application that helps you keep your system updated. Relevant information like stats and analytics are instantly created from any number of readily-available data sets, while team member task assignments can be as simple as drag-n-drop action. With CRM you get readable versions with useful charts featuring extensive data and customer histories. This is where a lot of software companies take pride, providing an upgrade from dreaded static spreadsheets, and complicated funnels/sales pipelines. There’s also an emphasis on the user-friendliness of today’s CRM. To streamline means to have all that essential data in one place-the ability to analyze, organize, and optimize-so that companies can paint their best customer behavior portrait. CRM achieves this by combining what could be several different programs into one single platform: customer communication, email marketing, phone, data logging and retrieval, reports and analytics, work task assignment, etc. “Streamline” is understood as making things faster and easier. OK, so, customer relationship management software is there to help streamline work for the sales team-or a number of other purposes such as social media management, leads/contacts organization, customer support, email campaigns, lead scoring, subscriptions, sign-ups, etc. Synthesis: Good CRM software should be a snap to set up and a piece of cake to learn. Thesis: CRM software saves your business time.Īntithesis: Setting up and learning how to use a CRM takes time. Let’s take a step back and approach the CRM question tangentially, using some good old fashioned dialectical reasoning: When looking at the circumstances of this helicopter in this way, it is clear that things could have been different if only the development time had been much shorter. Secondly, a new alternative to the aircraft’s reconnaissance tasks was developed in the form of unmanned airplanes, which are still used a lot for this purpose. These cut backs must be seen in the context of a time period in which the cold war was coming to an end. This led to a huge price increase per machine. First, during the development years, several governments cut back on the planned number of orders. As well as the 'function creep' already mentioned, there are other factors that also contributed to this failure. At the beginning of 2004, 13 years after the start of the project and with about 7 billion dollars spent, the US army pulled the plug on the project. To some this is one of the main reasons why the project was finally halted. This process took many years, during which the US army asked for new features to be incorporated into the design. The team developed an advanced helicopter with stealth properties and state-of-the-art sensor technology in order to fulfil its surveillance role well. Later, the objectives of the task were changed to deliver a surveillance helicopter. Development of the aircraft was commenced by the Boeing-Sikorsky collaboration when, in 1991, it won a US army contest to develop a light attack helicopter. Capable of pursuing and attacking others, this machine would have been outstanding in finding targets deep in the battle field and pointing them out to the Apache longbow attack helicopter. It should have been the invisible stealth craft that was able to surprise friend and foe. The same is true for the advanced and unfortunate Boeing-Sikorsky Comanche helicopter. The helicopter has been identified as a “complementary” program to the ArmyÃs Future Combat Systems, meaning it must effortlessly interface with and add to the capabilities of the equipment for the future, joint force.Every industry has its own failures. Four engineering and manufacturing development RAH-66 helicopters are in production as of January 2004, with the first to be flying in less than 18 months. The Army in late February 2004 terminated the Comanche program, and pledged to use the funds saved from the termination to implement modernization of existing helicopters and perhaps unmanned aerial vehicles. There is no known foreign counterpart to the next-generation helicopter. There are no foreign participants in the program or planned sales to other nations at this time. In October, 2002, restructuring the program, the PentagonÃs acquisition chief determined that the Army should buy 650 of the helicopters, with a production decision slated for the first quarter of 2010. The helicopter is expected to do this day and night and in bad weather using digital communications to be part of the future joint network of ground, air and space systems. The helicopter is designed to expand the ArmyÃs ability to conduct reconnaissance, deep, precision strike missions against time-sensitive targets and provide direct support to maneuvering forces. The Comanche is intended to replace the current fleet of Bell Helicopter Textron OH-58 helicopters in all air cavalry troops and light division attack helicopter battalions, and supplement the Boeing AH-64 Apache in heavy division/corps attack helicopter battalions. The armed reconnaissance Comanche helicopter is an all-composite, low-observable, twin-turbine, tandem two-seat helicopter with projected missions of armed reconnaissance, light attack and air combat. 1 Optimize Windows for better performance.1.1 Disable programs that run on startup.1.6 Turn on Windows 10 Fast Startup Feature.2 Switch to High-performance power plan.3.1 Upgrade to a faster drive Optimize Windows for better performance.If you want a quick boost in Windows 10 performance, try cleaning Windows 10 junk, Tweak system settings, and stop unwanted startup services. Install and run third-party free system optimizer like Ccleaner to clean junk, and cache and fix registry problems with one click.It can create a noticeable difference in window speed. Perform a full system scan with the latest updated antivirus software.Also, make sure your PC have the latest updates installed, or you can check for updates from windows settings, Update & Security.Start with windows vista and 7 Now Windows 10 also have some nice eye candy shadows, animations, and visual effects. Another reason for your PC to be running slow can be visual effects and animations that increase the burden on the system resources. On fast, newer PCs, these don’t usually affect system performance. Press Windows + R, type Sysdm.cpl hit the Enter key.īut on slower and older PCs, they can exact a performance hit.This will open system properties with several tabs.Click on the Advanced tab, and under Performance click Settings.Now On the Visual Effects tab, select Adjust for best performance.click apply and ok to make save changes.One of the biggest factors that slow down any Windows computer is unwanted software, aka bloatware. To remove bloatware or unused applications, follow these steps: They take up CPU, system resources, book disk and memory space unnecessarily hindering other applications to perform at their full potential. Press Windows + R, type appwiz.cpl and ok.This will Programs and Features and display all installed app list.Select the unused application and click Uninstall/Change to remove it.Follow the instructions in the uninstall dialog boxes to uninstall the software Run Disk Cleanup.Occasionally you may need to clean up your computer in order to get rid of old software, temporary objects, and junk files. Some software programs might not be completely uninstalled and the leftover files can cause performance issues. Press Windows Key + E and select this PC.Right-click on the C drive and select Properties.Select all items from the list box and then click OK.With Windows 10 there is a new “hybrid” startup mode Called Fast Startup, which helps in starting your PC faster after a shutdown by cutting down on boot-up time, using caching for some necessary resources into a single file on the hard disk. It takes up less space on your RAm than other memory optimizers, such as Razer Cortex.Finally, tick the box Turn on fast startup (recommended).Then click on Change settings that are currently unavailable.Click on Choose what the power buttons do.And at the time of startup, this master file is loaded back into the RAM speeds up the process manifolds. Running out of Random Access Memory (RAM) A large number of startup and background programs Fragmented or old hard drive Lack of updates on driver or Windows. Wise Memory Optimizer is user-friendly, making it easy for anyone. You can run this program on Windows OS only. Wise Memory Optimizer has working issues after system updates. Use light weight antivirus (meaning the antivirus which does not consume much memory.) If you are not using your computer for business purpose then use. If the scan determines that your data is not sorted how it should be, the program will start the defragmentation of the hard-disk. However, if you want it to keep track of your CPU performance all the time, you can adjust it to work all the time. This RAM memory optimizer is activated by command, meaning that it does not run in the background. The last thing you want in a program meant to speed up your computer is to have the process slow it down. Net is a Memory Optimizer it’s a smart memory management program that will keep your computer (PC) running better, faster, and longer. This might all be a result of an attempt to keep the application simple for resources sake, which makes a lot of sense. The interface is also very easy to navigate, and the simplicity is important for newcomers. The creators made a user-friendly software, which means you only need a basic level of computing knowledge to make it work. Wise Memory Optimizer is very tidy and well organized. This program is easy to use, and it’s a great way to make your computer feel new again. All these activities can slow down your machine to a point where simple games take ages to load. Your PC runs programs in the background, something we seldom pay attention to. Wise Memory Optimizer is a must-have software for any Windows computer. Wise Memory Optimizer eliminates programs and other things that slow down your computer. What does this terminal say? What does that message reveal about some character that’s long gone and whom I’ll never meet? But planet-hopping is fun, and I enjoyed seeing how the worlds differed.Įven as I often wanted to power through my run because of the muddled graphics and technical issues, I still couldn’t help myself from exploring every detail. The world, or worlds, are smaller than the maps of other popular open-world games, including The Legend of Zelda: Breath of the Wild and Witcher 3, which are both on Switch. The Outer Worlds brings biting wit in small details and dialogue, a wealth of characters to love and hate with equal passion, and an open world ripe for exploration. Obsidian, the developer behind The Outer Worlds, worked on those two Fallout titles, and it’s easy to see the connection. Fallout fans, especially those keen on Fallout 3 and Fallout: New Vegas, will love it. It’s not the best game I’ve ever played, even within the genre, but it’s a solid choice that delivers a decent story and doesn’t overstay its welcome. Still, its worth remembering that The Outer Worlds is a great game. They make it impossible to recommend the port. The technical issues are, on fact, insurmountable. Waiting 30 seconds to respawn if you die in the middle of that chaos makes the experience even worse. Melee combat left me swinging wildly at foes who just dodged a hair out of reach, while ranged combat was difficult due to the unresponsive frame rate. This is especially problematic during combat, on top of the distant rendering issues. The Switch port targets an unimpressive 30 frames per second, but even that feels spotty. But The Witcher 3: Wild Hunt and Bioshock Infinite managed to make the leap at an acceptable level of quality. Perhaps this is simply beyond what the Switch can handle. So why can’t The Outer Worlds?Īfter a while, I wondered if a game of this kind with scaled-down graphics might be asking too much. The Witcher 3: Wild Hunt and Bioshock Infinite did it. There’s also a great deal of sputtering and lag, especially when outside. You can easily wait up to 30 seconds each time you enter and exit your ship or a building, and when you travel. At more than 10 GB, it takes up more space than many other Switch titles, and there are seemingly endless loading screens that suggest a game worthy of such a long wait will emerge. Playing The Outer Worlds on the Switch should be impressive. I lost the sense of wonder associated with exploring a new world. Missing objects change the entire feel of the game, as I now found myself walking through a barren landscape rather than a lush planet. Entire objects and details like bricks, shrubs, or paths are completely gone. Just to be sure, I briefly revisited the game on the Xbox One X and PC to see if I was misremembering things. The near-constant pop-ins often took me out of the gameplay, and I was left frustrated. Besides being a major letdown, the poor visuals make it more challenging to engage in distant combat. Textures look amateur, and anything outside your immediate vicinity is a blurry mess. Still, I trekked on, convinced that I would find those psychedelic fields I was enamored with on my first playthrough on the Xbox One X. The Switch port failed to meet my expectations in numerous ways and, more importantly, it fails to do the game’s unique style justice.Īs I took my first steps on Terra 2, the introductory planet in The Outer Worlds, I could immediately tell this port would be rough. On the Switch, I felt like I was playing an entirely different game. Playing on an Xbox One X, I was amazed by its vibrant foliage, dazzling skies, and the endearing rust-buckets used as spaceships. What it lacked in character models or high-resolution textures, it made up for with wonderfully detailed planets and a cohesive look that underscored its snarky capitalist dystopia. The Outer Worlds was not the most visually impressive game when it came out in 2019, but it did have a stunning aesthetic. Though it’s still made for a pretty good Switch game. While this version isn’t an unplayable miss, which is more than can be said of some other ports, it certainly isn’t well done - and doesn’t do the original game justice. I thought it might have the polish that other Switch ports lacked, since it wasn’t trying to play catchup to the console’s success. News that The Outer Worlds would come to Nintendo’s incredibly popular Switch console left me cautiously optimistic. A perfect idea if you are finding a birthday gift, a housewarming gift, a festival gift, a Mother’s Day/ Father’s Day/ Christmas gift for your family or friends. FREE delivery Sat, May 20 on 25 of items shipped by Amazon. Gift of love: Whether you buy it for yourself or for your friend, family member, colleague, this keychain bracelet fits the need to be a very useful and beautiful gift, she can use it every day. 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If there isn't, I move on to the next bullet if there is I do further filtering by tile type to decide what to do (Tile A: bounce bullet, Tile B: destroy tile, Tile C: Destroy bullet & tile, etc.). I'm just testing each bullet once per tick and seeing if there is a tile at the same position. I just bypassed the 'on collision/is overlap' commands by rolling my own. All you can reliably collide with are the borders. It's almost like the interiors of the sub-sections are simply tiled backgrounds. I assumed that you could still tell when an object is inside on of those squares, but apparently you can't. Ashley explained how tilemaps are automatically broken into square sub-sections when you have large areas full of the same kind of tiles. Example: Is overlapping will come up 'no'.Ģ. If the bullet moves too far into a tilemap section without destroying the tile or itself, you can't get collision information. CAPTAIN VIJAYAKANTH DODGING THE BULLET IN DHARMAPURI Tags : Captain, Vijayakanth Tweet FACEBOOK COMMENTS OTHER LATEST SLIDE SHOWS Vijay Bday Special: People's choice - The 10 Thalapathy. Using 'Is overlapping?' and 'On Collision' does not seem to be reliable for tilemaps when fast moving objects - bullets - are in play. I suppose its similar to how we all still mention how Superman is "able to leap tall buildings in a single bound" despite the fact that he's been more inclined to fly than leap for the vast majority of his history.I've just been dealing with this in my own game, transitioning from a simple roll-your-own tilemap system based around objects to a proper tilemap. Why would she need bulletproof bracelets if her skin is itself bulletproof? Why does she need an invisible jet if she can fly under her own power? But because those things were iconic, they weren't entirely forgotten about. Her powers have gone up and down since then, but generally hew more closely to the invulnerable side of things than her classic power set.Īs a result of those changes, a number of iconic bits of her costume and gadgets from the past became more or less redundant. When George Perez rebooted the character post- Crisis on Infinite Earths, her powers were amped up considerably, to the point where she was only slightly less powerful than Superman. If you shot at her with a decently powered gun, it would most likely do significant damage to her. She was stronger, faster, tougher, and more resilient than the average human, but not massively so. Throughout the Golden and Silver Ages, she was basically just human-plus. Join on Discord | Follow on Twitter | Newbie Guide | Recommended Reading | Milestone FAQĭiana's powers have changed significantly over the years. R/DCcomics News Artwork Comics Fan Made Film + TV Cosplay Merchandise Video Games Webcomics Other Recommendations Spoiler Free Related Subreddits Comics Justice League Teen Titans Watchmen Major Events New 52 Rebirth TV Movies Video Games DC For All Ages Vertigo Wildstorm ago Wasn't Diana shot in the back in Rucka's Rebirth run Smallgenie549 5 yr. Looking for something to read? Try one of these 100+ recommendations, all chosen by r/DCcomics users.Īquaman Batman Flash Green Arrow Green Lantern Superman Wonder Woman Other It’s quite an old weakness but it’s been fairly consistent over the years, things like bullets and blades could kill her but it would be nearly impossible to beat her to death with blunt force. If you are submitting a link, do not include the spoiler in your submitted link name. If a significant event has taken place within one year of its release, mark it as a spoiler. Now aim up at the platform and try to shoot at an angle so that the bullet hits the 2nd player after the 6th bounce.
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